Cellular structural and functional imaging of donor and pathological corneas with label-free dual-mode full-field optical coherence tomography

作者：Keyi Fei, Zhongzhou Luo, Yu‐Pei Chen, Yuancong Huang, Saiqun Li, Viacheslav Mazlin, Claude Boccara, Jin Yuan, Peng Xiao · 发表于：Biomedical Optics Express · 年份：2024 · DOI：10.1364/boe.525116 · 被引用次数：14 · 研究领域：Corneal surgery and disorders、Corneal Surgery and Treatments、Glaucoma and retinal disorders

In this study, a dual-mode full-field optical coherence tomography (FFOCT) was customized for label-free static and dynamic imaging of corneal tissues, including donor grafts and pathological specimens. Static images effectively depict relatively stable structures such as stroma, scar, and nerve fibers, while dynamic images highlight cells with active intracellular metabolism, specifically for corneal epithelial cells. The dual-mode images complementarily demonstrate the 3D microstructural features of the cornea and limbus. Dual-modal imaging reveals morphological and functional changes in corneal epithelial cells without labeling, indicating cellular apoptosis, swelling, deformation, dynamic signal alterations, and distinctive features of inflammatory cells in keratoconus and corneal leukoplakia. These findings propose dual-mode FFOCT as a promising technique for cellular-level cornea and limbus imaging.