Eubacterium album sp. nov., a butyrate-producing bacterium isolated from faeces of a healthy human

作者：Feng-Lan Liu, Rashidin Abdugheni, Cong-Guo Ran, Nan Zhou, Shuang‐Jiang Liu · 发表于：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · 年份：2024 · DOI：10.1099/ijsem.0.006380 · 被引用次数：5 · 研究领域：Genomics and Phylogenetic Studies、Probiotics and Fermented Foods、Gut microbiota and health

An oval to rod-shaped, Gram-stain-positive, strictly anaerobic bacterium, designated LFL-14 T , was isolated from the faeces of a healthy Chinese woman. Cells of the strain were non-spore-forming, grew optimally at 37 °C (growth range 30–45 °C) and pH 7.0 (growth range 6.0–9.0) under anaerobic conditions in the liquid modified Gifu anaerobic medium (mGAM). The result of 16S rRNA gene-based analysis indicated that LFL-14 T shared an identity of 94.7 0% with Eubacterium ventriosum ATCC 27560 T , indicating LFL-14 T represented a novel taxon. The results of genome-based analysis revealed that the average nucleotide identity (ANI), the digital DNA–DNA hybridisation (dDDH) and average amino acid identity (AAI) between LFL-14 T and its phylogenetically closest neighbour, Eubacterium ventriosum ATCC 27560 T , were 77.0 %, 24.6 and 70.9 %, respectively, indicating that LFL-14 T represents a novel species of the genus Eubacterium . The genome size of LFL-14 T was 2.92 Mbp and the DNA G+C content was 33.14 mol%. We analysed the distribution of the genome of LFL-14 T in cohorts of healthy individuals, type 2 diabetes patients (T2D) and patients with non-alcoholic fatty liver disease (NAFLD). We found that its abundance was higher in the T2D cohort, but it had a low average abundance of less than 0.2 % in all three cohorts. The percentages of frequency of occurrence in the T2D, healthy and NAFLD cohorts were 48.87 %, 16.72 % and 13.10 % respectively. The major cellular fatty acids of LFL...