Disposable Peptidoglycan-Specific Biosensor for Noninvasive Real-Time Detection of Broad-Spectrum Gram-Positive Bacteria in Exhaled Breath Condensates

作者：Jian Zhang, Haochen Qi, Jie Wu, Xuanjiao Mao, Hailin Zhang, Niloufar Amin, Feng Xu, Changkun Dong, Chunchang Wang, Pengjun Wang, Lei Zheng · 发表于：Analytical Chemistry · 年份：2024 · DOI：10.1021/acs.analchem.4c00059 · 被引用次数：46 · 研究领域：Advanced Chemical Sensor Technologies、Acoustic Wave Resonator Technologies、Biosensors and Analytical Detection

Rapidly identifying and quantifying Gram-positive bacteria are crucial to diagnosing and treating bacterial lower respiratory tract infections (LRTIs). This work presents a field-deployable biosensor for detecting Gram-positive bacteria from exhaled breath condensates (EBCs) based on peptidoglycan recognition using an aptamer. Dielectrophoretic force is employed to enrich the bacteria in 10 s without additional equipment or steps. Concurrently, the measurement of the sensor’s interfacial capacitance is coupled to quantify the bacteria during the enrichment process. By incorporation of a semiconductor condenser, the whole detection process, including EBC collection, takes about 3 min. This biosensor has a detection limit of 10 CFU/mL, a linear range of up to 10 5 CFU/mL and a selectivity of 1479:1. It is cost-effective and disposable due to its low cost. The sensor provides a nonstaining, culture-free and PCR-independent solution for noninvasive and real-time diagnosis of Gram-positive bacterial LRTIs.