Diversity and host interaction of the gut microbiota in specific pathogen-free pigs

作者：Mingxing Wen, Shuangshuang Chen, Yali Zhang, Yan Liu, Chuang Tang, Jinwei Zhang, Jing Sun, Xiaokai Li, Yuchun Ding, Lu Lu, Keren Long, Yong Nie, Xuewei Li, Mingzhou Li, Liangpeng Ge, Jideng Ma · 发表于：Frontiers in Microbiology · 年份：2024 · DOI：10.3389/fmicb.2024.1402807 · 被引用次数：10 · 研究领域：Gut microbiota and health、Clostridium difficile and Clostridium perfringens research、Helicobacter pylori-related gastroenterology studies

Pigs are widely used as animal models in various studies related to humans. The interaction between the gut microbiota and the host has significant effects on the host’s health and disease status. However, although there have been many studies investigating the pig gut microbiota, the findings have been inconsistent due to variations in rearing conditions. Interactions between the gut microbiota and host have not been fully explored in pigs. Specific pathogen-free (SPF) pigs are ideal non-primate large animals to study the interactions between the gut microbiota and the host. In this study, we performed high-throughput sequencing analysis of the gut microbiota and the gut tissue transcriptome of six SPF pigs to provide a systematic understanding of the composition, function, and spatial distribution of gut microbiota in SPF pigs. We identified significant differences in microbial diversity and functionality among different gastrointestinal tract sites. Metagenomics data analysis revealed significant differences in alpha diversity and beta diversity of microbiota in different gastrointestinal sites of SPF pigs. Additionally, transcriptomic data indicated significant differences in gene expression as well as KEGG and GO functional enrichment between the small intestine and large intestine. Furthermore, by combining microbial metagenomics and host transcriptomics analyses, specific correlations were found between gut microbiota and host genes. These included a negative correlati...