Effects and microbiota changes following oral lyophilized fecal microbiota transplantation in children with autism spectrum disorder

作者：Youran Li, Pei Xiao, Rong Cao, Jun Le, Qiao Xu, Fangfei Xiao, Lin Ye, Xufei Wang, Yizhong Wang, Ting Zhang · 发表于：Frontiers in Pediatrics · 年份：2024 · DOI：10.3389/fped.2024.1369823 · 被引用次数：28 · 研究领域：Gut microbiota and health、Gastrointestinal motility and disorders、Clostridium difficile and Clostridium perfringens research

Background and purpose Autism spectrum disorder (ASD) is a group of heterogeneous neurodevelopmental disorders that is characterized by core features in social communication impairment and restricted, repetitive sensory-motor behaviors. This study aimed to further investigate the utilization of fecal microbiota transplantation (FMT) in children with ASD, both with and without gastrointestinal (GI) symptoms, evaluate the effect of FMT and analyze the alterations in bacterial and fungal composition within the gut microbiota. Methods A total of 38 children diagnosed with ASD participated in the study and underwent oral lyophilized FMT treatment. The dosage of the FMT treatment was determined based on a ratio of 1 g of donor stool per 1 kg of recipient body weight, with a frequency of once every 4 weeks for a total of 12 weeks. In addition, 30 healthy controls (HC) were included in the analysis. The clinical efficacy of FMT was evaluated, while the composition of fecal bacteria and fungi was determined using 16S rRNA and ITS gene sequencing methods. Results Median age of the 38 children with ASD was 7 years. Among these children, 84.2% (32 of 38) were boys and 81.6% (31 of 38) exhibited GI symptoms, with indigestion, constipation and diarrhea being the most common symptoms. Sample collections and assessments were conducted at baseline (week 0), post-treatment (week 12) and follow-up (week 20). At the end of the follow-up phase after FMT treatment, the autism behavior checklist (A...