Effect on the splenocyte function of weaned piglets induced by continuous lipopolysaccharide injections

作者：Tingyu Yang, Guotong Zhao, Wenlu Zhu, Wanting Yu, Yijie Jiang, Yunxiao Zhou, Yong Li · 发表于：Journal of Veterinary Research · 年份：2024 · DOI：10.2478/jvetres-2024-0024 · 被引用次数：1 · 研究领域：Immune Response and Inflammation、Immune cells in cancer、Immune responses and vaccinations

Abstract Introduction When piglets are exposed to pathogens for a long period, the immune system organs, among them the spleen, play a major role in combating the stress caused by those pathogens. In the present study, the effect on splenocyte function was investigated in a model of weaned piglets in which stress was induced by multiple low doses of lipopolysaccharide (LPS). Material and Methods Forty-eight 28-day-old piglets were divided into two groups: the LPS group and the control group. During the experimental period of thirteen days, the LPS group was intraperitoneally injected with LPS (100 μg/kg) once per day, and the control group was injected with the same volume of 0.9% sterile saline. On the 1 st , 5 th , 9 th and 13 th days, the piglets’ spleens were collected for isolating splenocytes. The proliferation ability of splenocytes was evaluated by the cell-counting-kit 8 method. Flow cytometry was used to detect cell cycle stage and apoptosis, and the nitric oxide level of cell supernatant was also tested. Results In the experimental group, the proliferation ability of splenocytes was enhanced, the proportion of cells in the G0/G1 phase was smaller, and cells were promoted to the S and G2/M phases. Meanwhile, apoptosis was suppressed and nitric oxide release upregulated. The results were significantly different between the LPS group and the control group on the 5 th and 9 th days. Conclusion The difference between the results of one group and those of the other sugge...