Christensenella strain resources, genomic/metabolomic profiling, and association with host at species level

作者：Xin‐Wei Sun, Haojie Huang, Xiaomeng Wang, Rui-Qi Wei, Han-Yu Niu, Hao Yu Chen, Man Luo, Rashidin Abdugheni, Yulin Wang, Feng-Lan Liu, He Jiang, Chang Liu, Shuang‐Jiang Liu · 发表于：Gut Microbes · 年份：2024 · DOI：10.1080/19490976.2024.2347725 · 被引用次数：39 · 研究领域：Microbial Community Ecology and Physiology、Gut microbiota and health、Probiotics and Fermented Foods

The gut commensal bacteria Christensenellaceae species are negatively associated with many metabolic diseases, and have been seen as promising next-generation probiotics. However, the cultured Christensenellaceae strain resources were limited, and their beneficial mechanisms for improving metabolic diseases have yet to be explored. In this study, we developed a method that enabled the enrichment and cultivation of Christensenellaceae strains from fecal samples. Using this method, a collection of Christensenellaceae Gut Microbial Biobank (ChrisGMB) was established, composed of 87 strains and genomes that represent 14 species of 8 genera. Seven species were first described and the cultured Christensenellaceae resources have been significantly expanded at species and strain levels. Christensenella strains exerted different abilities in utilization of various complex polysaccharides and other carbon sources, exhibited host-adaptation capabilities such as acid tolerance and bile tolerance, produced a wide range of volatile probiotic metabolites and secondary bile acids. Cohort analyses demonstrated that Christensenellaceae and Christensenella were prevalent in various cohorts and the abundances were significantly reduced in T2D and OB cohorts. At species level, Christensenellaceae showed different changes among healthy and disease cohorts. C. faecalis, F. tenuis, L. tenuis, and Guo. tenuis significantly reduced in all the metabolic disease cohorts. The relative abundances of C. mi...