Acinetobacter baumannii biofilm was inhibited by tryptanthrin through disrupting its different stages and genes expression

作者：Tingting Guo, Na Zhou, Liying Yang, Zichen Wang, Changchao Huan, Tao Lin, Guangyu Bao, Jian Hu, Guocai Li · 发表于：iScience · 年份：2024 · DOI：10.1016/j.isci.2024.109942 · 被引用次数：14 · 研究领域：Antibiotic Resistance in Bacteria、Antimicrobial Peptides and Activities、Antimicrobial agents and applications

Biofilm formation plays a significant role in antibiotic resistance, necessitating the search for alternative therapies against biofilm-associated infections. This study demonstrates that 20 μg/mL tryptanthrin can hinder biofilm formation above 50% in various A. baumannii strains. Tryptanthrin impacts various stages of biofilm formation, including the inhibition of surface motility and eDNA release in A. baumannii , as well as an increase in its sensitivity to H 2 0 2 . RT-qPCR analysis reveals that tryptanthrin significantly decreases the expression of the following genes: abaI (19.07%), abaR (33.47%), bfmR (43.41%), csuA/B (64.16%), csuE (50.20%), ompA (67.93%), and katE (72.53%), which are related to biofilm formation and quorum sensing. Furthermore, tryptanthrin is relatively safe and can reduce the virulence of A. baumannii in a Galleria mellonella infection model. Overall, our study demonstrates the potential of tryptanthrin in controlling biofilm formation and virulence of A. baumannii by disrupting different stages of biofilm formation and intercellular signaling communication.