Screening of Cross-Reactive Aptamers for the Detection of 24 Quinolones by Using a Liebig’s Law-Guided Parallel-Series Strategy

作者：Weijuan Yang, Dongdong Luo, Siyu Zheng, Yiru Zhang, Zongwen Wang, FengFu Fu · 发表于：Analytical Chemistry · 年份：2024 · DOI：10.1021/acs.analchem.4c00616 · 被引用次数：11 · 研究领域：Advanced biosensing and bioanalysis techniques、Antibiotics Pharmacokinetics and Efficacy、Biosensors and Analytical Detection

Quinolones, a widely used class of antibiotics, present significant environmental and health concerns if they excessively remain in the environment and in food. Aptamers specific to quinolones can be applied as bioreceptors for the detection of quinolone residues in the environment and food. The quinolone family contains dozens of different individuals that share the same core structure coupled with various substituents at six different positions. The diversity and complexity of the substitution sites make it a challenge to choose a set of representative molecules that encompass all the desired sites and preserve the core molecular framework for the screening of quinolone-specific aptamers via systematic evolution of ligands by exponential enrichment (SELEX). To address this challenge, we introduce a novel parallel-series strategy guided by Liebig's law for isolating quinolone-specific cross-reactive aptamers by using the library-immobilized SELEX method. Through this approach, we successfully identified 5 aptamers (Apt.AQ01-Apt.AQ05) with high binding affinity and excellent specificity to 24 different quinolone individuals. Among them, Apt.AQ03 showcased optimal performance with affinities ranging from 0.14 to 1.07 μM across the comprehensive set of 24 quinolones, exhibiting excellent specificity against nontarget interferents. The binding performance of Apt.AQ03 was further characterized with microscale thermophoresis, circular dichroism spectra, and an exonuclease digestio...