Gastrodin regulates the expression of renin‐angiotensin system–SIRT3 and proinflammatory mediators in reactive astrocytes via activated microglia

作者：Hanjun Zuo, Xue‐Qi Ren, Jin‐Sha Shi, Hao‐Long Shi, Kun Guo, Pengxiang Wang, Min Zhao, Juan‐Juan Li · 发表于：European Journal of Neuroscience · 年份：2024 · DOI：10.1111/ejn.16371 · 被引用次数：5 · 研究领域：Biological and pharmacological studies of plants、Neuroinflammation and Neurodegeneration Mechanisms、Neuroendocrine regulation and behavior

Abstract Gastrodin, an anti‐inflammatory herbal agent, is known to suppress microglia activation. Here, we investigated whether it would exert a similar effect in reactive astrocytes and whether it might act through the renin‐angiotensin system (RAS) and sirtuin 3 (SIRT3). Angiotensinogen (ATO), angiotensin‐converting enzyme (ACE), angiotensin II type 1 (AT 1 ) and type 2 (AT 2 ) receptor and SIRT3 expression was detected in TNC‐1 astrocytes treated with BV‐2 microglia conditioned medium (CM) with or without gastrodin and lipopolysaccharide (LPS) pre‐treatment by RT‐PCR, immunofluorescence and western blotting analysis. Expression of C3 (A1 astrocyte marker), S100A10 (A2 astrocyte marker), proinflammatory cytokines and neurotrophic factors was then evaluated. The results showed a significant increase of ATO, ACE, AT 1 , SIRT3, C3, proinflammatory cytokines and neurotrophic factors expression in TNC‐1 astrocytes incubated in CM + LPS when compared with cells incubated in the CM, but AT 2 and S100A10 expression was reduced. TNC‐1 astrocytes responded vigorously to BV‐2 CM treated with gastrodin + LPS as compared with the control. This was evident by the decreased expression of the abovementioned protein markers, except for AT 2 and S100A10. Interestingly, SIRT3, IGF‐1 and BDNF expression was enhanced, suggesting that gastrodin inhibited the expression of RAS and proinflammatory mediators but promoted the expression of neurotrophic factors. And gastrodin regulated the phenotypic...