Correlation of gasdermin B staining patterns with prognosis, progression, and immune response in colorectal cancer

作者：Liang Sun, Jiahui Wang, Yuxuan Li, Yixin Kang, Yi Jiang, Jun Zhang, Senmi Qian, Fangying Xu · 发表于：BMC Cancer · 年份：2024 · DOI：10.1186/s12885-024-12326-2 · 被引用次数：12 · 研究领域：Inflammasome and immune disorders、Autophagy in Disease and Therapy、Immune cells in cancer

Abstract Background Pyroptosis is a type of programmed cell death mediated by the gasdermin family. Gasdermin B (GSDMB), as a member of gasdermin family, can promote the occurrence of cell pyroptosis. However, the correlations of the GSDMB expression in colorectal cancer with clinicopathological predictors, immune microenvironment, and prognosis are unclear. Methods Specimens from 267 colorectal cancer cases were analyzed by immunohistochemistry to determine GSDMB expression, CD3 + , CD4 + , and CD8 + T lymphocytes, CD20 + B lymphocytes, CD68 + macrophages, and S100A8 + immune cells. GSDMB expression in cancer cells was scored in the membrane, cytoplasm, and nucleus respectively. GSDMB + immune cell density was calculated. Univariate and multivariate survival analyses were performed. The association of GSDMB expression with other clinicopathological variables and immune cells were also analyzed. Double immunofluorescence was used to identify the nature of GSDMB + immune cells. Cytotoxicity assays and sensitivity assays were performed to detect the sensitivity of cells to 5-fluorouracil. Results Multivariate survival analysis showed that cytoplasmic GSDMB expression was an independent favorable prognostic indicator. Patients with positive cytoplasmic or nuclear GSDMB expression would benefit from 5-fluorouracil based chemotherapy. The assays in vitro showed that high GSDMB expression enhanced the sensitivity of colorectal cancer cells to 5-fluorouracil. Patients with positive ...