Detection of clonal plasma cells in POEMS syndrome using multiparameter flow cytometry

作者：Arata Ishii, Shokichi Tsukamoto, Naoya Mimura, Yurie Miyamoto‐Nagai, Yusuke Isshiki, Shinichiro Matsui, Sanshiro Nakao, Asuka Shibamiya, Yutaro Hino, Kensuke Kayamori, Nagisa Oshima‐Hasegawa, Tomoya Muto, Yusuke Takeda, Tomoki Suichi, Sonoko Misawa, Chikako Ohwada, Koutaro Yokote, Satoshi Kuwabara, Chiaki Nakaseko, Hiroyuki Takamatsu, Emiko Sakaida · 发表于：Scientific Reports · 年份：2024 · DOI：10.1038/s41598-024-61034-1 · 被引用次数：2 · 研究领域：Peripheral Neuropathies and Disorders、Multiple Myeloma Research and Treatments、Platelet Disorders and Treatments

Abstract POEMS syndrome (polyneuropathy, organomegaly, endocrinopathy, monoclonal protein [M-protein], and skin changes) is a rare systemic disorder characterized by various symptoms caused by underlying plasma cell (PC) dyscrasia. Detection of monoclonal PCs is mandatory for the diagnosis of POEMS syndrome; however, the usefulness of EuroFlow-based next-generation flow cytometry (EuroFlow-NGF) in POEMS syndrome for detecting monoclonal PCs in bone marrow (BM) and the gating strategy suitable for flow cytometry study of POEMS syndrome remain unknown. We employed EuroFlow-NGF-based single-tube eight-color multiparameter flow cytometry (MM-flow) and established a new gating strategy (POEMS-flow) to detect the monoclonal PCs in POEMS syndrome, gating CD38 broadly from dim to bright and CD45 narrowly from negative to dim compared to MM-flow. MM-flow detected monoclonal PCs in 9/25 (36.0%) cases, including 2/2 immunofixation electrophoresis (IFE)-negative cases (100%). However, POEMS-flow detected monoclonal PCs in 18/25 cases (72.0%), including 2/2 IFE-negative cases (100%). POEMS-flow detected monoclonal PCs with immunophenotypes of CD19 − in 17/18 (94.4%). In six cases where post-treatment samples were available, the size of the clones was significantly reduced after the treatment ( P = 0.031). POEMS-flow can enhance the identification rate of monoclonal PCs in POEMS syndrome and become a valuable tool for the diagnosis of POEMS syndrome.