DNA Reaction Circuits to Establish Designated Biological Functions in Multicellular Community

作者：Qiang Zhang, Yue Zhang, Limei Wu, Dan Wang, Yuting Zhuo, Lu Yao, Yue Liu, Zhimin Wang, Liping Qiu, Weihong Tan · 发表于：Nano Letters · 年份：2024 · DOI：10.1021/acs.nanolett.4c00980 · 被引用次数：11 · 研究领域：Advanced biosensing and bioanalysis techniques、DNA and Nucleic Acid Chemistry、RNA Interference and Gene Delivery

In multicellular organisms, individual cells are coordinated through complex communication networks to accomplish various physiological tasks. Aiming to establish new biological functions in the multicellular community, we used DNA as the building block to develop a cascade of nongenetic reaction circuits to establish a dynamic cell-cell communication network. Utilizing membrane-anchored amphiphilic DNA tetrahedra (TDN) as the nanoscaffold, reaction circuits were incorporated into three unrelated cells in order to uniquely regulate their sense-and-response behaviors. As a proof-of-concept, this step enabled these cells to simulate significant biological events involved in T cell-mediated anticancer immunity. Such events included cancer-associated antigen recognition and the presentation of antigen-presenting cells (APCs), APC-facilitated T cell activation and dissociation, and T cell-mediated cancer targeting and killing. By combining the excellent programmability and molecular recognition ability of DNA, our cell-surface reaction circuits hold promise for mimicking and manipulating many biological processes.