Development of real‐time recombinase polymerase amplification ( RPA ) and RPA combined with lateral flow dipstick ( LFD ) assays for the rapid and sensitive detection of cyprinid herpesvirus 3

作者：Yingying Li, Ruifan Li, Xubing Mo, Yingying Wang, Jiyuan Yin, Sven Bergmann, Yan Ren, Houjun Pan, Cunbin Shi, Defeng Zhang, Qing Wang · 发表于：Journal of Fish Diseases · 年份：2024 · DOI：10.1111/jfd.13960 · 被引用次数：6 · 研究领域：Aquaculture disease management and microbiota、Herpesvirus Infections and Treatments、Biosensors and Analytical Detection

In this issue, we established rapid, cost-effective, and simple detection methods including recombines polymerase amplification with lateral flow dipstick (RPA-LFD) and real-time RPA for cyprinid herpesvirus 3(CyHV-3), and evaluated their sensitivity, specificity, and applicability, the real-time RPA method could achieve sensitive diagnosis of CyHV-3 within 1.3 copies per reaction, respectively. The real-time RPA method is 10-fold more sensitive than RPA-LFD method. The exact number of CyHV-3 can be calculated in each sample by real-time RPA. The sera from koi also can be tested in these methods. In addition, no cross-reaction was observed with other related pathogens, including carp oedema virus (CEV), spring viraemia of carp virus (SVCV), cyprinid herpesvirus 1(CyHV-1), cyprinid herpesvirus 2(CyHV-2), type I grass carp reovirus (GCRV-I), type II GCRV (GCRV-II), type III GCRV (GCRV-III), and Aeromonas hydrophila.