A novel variant in Salmonella genomic island 1 of multidrug-resistant Salmonella enterica serovar Kentucky ST198

作者：Rattanaporn Intuy, Sirirak Supa-amornkul, Bharkbhoom Jaemsai, Wuthiwat Ruangchai, Witthawat Wiriyarat, Soraya Chaturongakul, Prasit Palittapongarnpim · 发表于：Microbiology Spectrum · 年份：2024 · DOI：10.1128/spectrum.03994-23 · 被引用次数：6 · 研究领域：Salmonella and Campylobacter epidemiology、Antibiotic Resistance in Bacteria、Bacteriophages and microbial interactions

ABSTRACT Salmonella enterica serovar Kentucky ST198 is a major health threat due to its resistance to ciprofloxacin and several other drugs, including third-generation cephalosporins. Many drug-resistant genes have been identified in the Salmonella genomic island 1 variant K (SGI1-K). In this study, we investigated the antimicrobial resistance (AMR) profile and genotypic relatedness of two isolates of ciprofloxacin-resistant (CIP R ) S . Kentucky ST198 from poultry in Northeastern Thailand. We successfully assembled the complete genomes of both isolates, namely SSSE-01 and SSSE-03, using hybrid de novo assembly of both short- and long-read sequence data. The complete genomes revealed their highly similar genomic structures and a novel variant of SGI1-K underlying multidrug-resistant (MDR) patterns, including the presence of bla TEM-1b , which confers resistance to beta-lactams, including cephalosporins and lnu (F) which confers resistance to lincomycin and other lincosamides. In addition, the chromosomal mutations in the quinolone resistance-determining region (QRDR) were found at positions 83 (Ser83Phe) and 87 (Asp87Asn) of GyrA and at positions 57 (Thr57Ser) and 80 (Ser80Ile) of ParC suggesting high resistance to ciprofloxacin. We also compared SSSE-01 and SSSE-03 with publicly available complete genome data and revealed significant variations in SGI1-K genetic structures and variable relationships to antibiotic resistance. In comparison to the other isolates, SGI1-K of SSS...