Protocol for a comprehensive pipeline to study ancient human genomes

作者：Kongyang Zhu, Haifeng He, Le Tao, Hao Ma, Xiaomin Yang, Rui Wang, Jianxin Guo, Chuan‐Chao Wang · 发表于：STAR Protocols · 年份：2024 · DOI：10.1016/j.xpro.2024.102985 · 被引用次数：17 · 研究领域：Forensic and Genetic Research、Race, Genetics, and Society、Yersinia bacterium, plague, ectoparasites research

Ancient genomics has revolutionized our understanding of human evolution and migration history in recent years. Here, we present a protocol to prepare samples for ancient genomics research. We describe steps for releasing DNA from human remains, DNA library construction, hybridization capture, quantification, and sequencing. We then detail procedures for mapping sequence reads and population genetics analysis. This protocol also outlines challenges in extracting ancient DNA samples and authenticating ancient DNA to uncover the genetic history and diversity of ancient populations. For complete details on the use and execution of this protocol, please refer to Tao et al.1