Plants attacked above-ground by leaf-mining flies change below-ground microbiota to enhance plant defense

作者：Yang Gao, Qiong Yang, Qiulin Chen, Yunchuan He, Wei He, Jiamei Geng, Yunzeng Zhang, Ying Zhou, Zeng‐Rong Zhu · 发表于：Horticulture Research · 年份：2024 · DOI：10.1093/hr/uhae121 · 被引用次数：16 · 研究领域：Plant Parasitism and Resistance、Insect symbiosis and bacterial influences、Dengue and Mosquito Control Research

Abstract Root-associated microbiomes play a crucial role in plant responses to biotic and abiotic stresses. Plants can enrich beneficial microbes to increase their stress-relieving ability. Above-ground insect herbivory is among the most detrimental stresses for plants, especially to crop production. However, few studies have explored how root-associated microbiomes respond to herbivores and influence plant-defense functions under herbivory stress. We investigate the changes and functional role of root-associated microbial communities under herbivory stress using leafminer (Liriomyza trifolii) and cowpea (Vigna unguiculata) as a focal system. We did this by using a combination of 16S ribosomal RNA gene profiling and metagenomic sequencing to test for differences in co-occurrence networks and functions between cowpea plants infested and noninfested with leafminers. The results demonstrated that leafminer infestation caused a shift in the rhizosphere microbiome, which was characterized by a significant variation in microbiome community structure and composition, the selection of hub microbes involved in nitrogen (N) metabolism, and functional enrichment related to N metabolism. Notably, nitrogen-fixing bacteria Bradyrhizobium species were actively enriched and selected to be hubs in the rhizosphere. Inoculation with Bradyrhizobium enhanced cowpea performance under leafminer stress and increased protease inhibitor levels to decrease leafminer fitness. Overall, our study characte...