A 700 nm LED Light Activated Ru(II) Complex Destroys Tumor Cytoskeleton via Photosensitization and Photocatalysis

作者：Anyi Dao, Shiyan Chen, Pan Li, Qingyan Ren, Xun Wang, Haorui Wu, Qiufang Gong, Zeduan Chen, Shaomin Ji, Jiaxi Ru, HaoTu Zhu, Chao Liang, Pingyu Zhang, Haiping Xia, Huaiyi Huang · 发表于：Advanced Healthcare Materials · 年份：2024 · DOI：10.1002/adhm.202400956 · 被引用次数：24 · 研究领域：Nanoplatforms for cancer theranostics、Nanocluster Synthesis and Applications、Luminescence and Fluorescent Materials

Photoactivable chemotherapy (PACT) using metallic complexes provides spatiotemporal selectivity over drug activation for targeted anticancer therapy. However, the poor absorption in near-infrared (NIR) light region of most metallic complexes renders tissue penetration challenging. Herein, an NIR light triggered dinuclear photoactivable Ru(II) complex (Ru2) is presented and the antitumor mechanism is comprehensively investigated. The introduction of a donor-acceptor-donor (D-A-D) linker greatly enhances the intramolecular charge transition, resulting in a high molar extinction coefficient in the NIR region with an extended triplet excited state lifetime. Most importantly, when activated by 700 nm NIR light, Ru2 exhibits unique slow photodissociation kinetics that facilitates synergistic photosensitization and photocatalytic activity to destroy diverse intracellular biomolecules. In vitro and in vivo experiments show that when activated by 700 nm NIR light, Ru2 exhibits nanomolar photocytotoxicity toward 4T1 cancer cells via the induction of calcium overload and endoplasmic reticulum (ER) stress. These findings provide a robust foundation for the development of NIR-activated Ru(II) PACT complexes for phototherapeutic application.