Low-intensity pulsed ultrasound promotes the osteogenesis of mechanical force-treated periodontal ligament cells via Piezo1

作者：Fu Zheng, Tong Wu, Feifei Wang, Huazhi Li, Hongyi Tang, Xinyu Cui, Cuiying Li, Yi-Xiang Wang, Jiuhui Jiang · 发表于：Frontiers in Bioengineering and Biotechnology · 年份：2024 · DOI：10.3389/fbioe.2024.1347406 · 被引用次数：19 · 研究领域：Ultrasound and Hyperthermia Applications、Erythrocyte Function and Pathophysiology、Planarian Biology and Electrostimulation

Background Low-intensity pulsed ultrasound (LIPUS) can accelerate tooth movement and preserve tooth and bone integrity during orthodontic treatment. However, the mechanisms by which LIPUS affects tissue remodeling during orthodontic tooth movement (OTM) remain unclear. Periodontal ligament cells (PDLCs) are pivotal in maintaining periodontal tissue equilibrium when subjected to mechanical stimuli. One notable mechano-sensitive ion channel, Piezo1, can modulate cellular function in response to mechanical cues. This study aimed to elucidate the involvement of Piezo1 in the osteogenic response of force-treated PDLCs when stimulated by LIPUS. Method After establishing rat OTM models, LIPUS was used to stimulate rats locally. OTM distance and alveolar bone density were assessed using micro-computed tomography, and histological analyses included hematoxylin and eosin staining, tartrate-resistant acid phosphatase staining and immunohistochemical staining. GsMTx4 and Yoda1 were respectively utilized for Piezo1 functional inhibition and activation experiments in rats. We isolated human PDLCs (hPDLCs) in vitro and evaluated the effects of LIPUS on the osteogenic differentiation of force-treated hPDLCs using real-time quantitative PCR, Western blot, alkaline phosphatase and alizarin red staining. Small interfering RNA and Yoda1 were employed to validate the role of Piezo1 in this process. Results LIPUS promoted osteoclast differentiation and accelerated OTM in rats. Furthermore, LIPUS a...