Transmission of bla NDM in Enterobacteriaceae among animals, food and human

作者：Bo Fu, Jian Xu, Dandan Yin, Chengtao Sun, Dejun Liu, Weishuai Zhai, Rina Bai, Yue Cao, Qin Zhang, Shizhen Ma, Timothy R. Walsh, Fupin Hu, Yang Wang, Congming Wu, Jianzhong Shen · 发表于：Emerging Microbes & Infections · 年份：2024 · DOI：10.1080/22221751.2024.2337678 · 被引用次数：31 · 研究领域：Antibiotic Resistance in Bacteria、Antibiotics Pharmacokinetics and Efficacy、Pharmaceutical and Antibiotic Environmental Impacts

Despite carbapenems not being used in animal, Carbapenem-Resistant-Enterobacterales (CRE), particularly New-Delhi-metallo-β-lactamase-producing-CRE (NDM-CRE), are prevalent in livestock. Concurrently, the incidence of human infections caused by NDM-CRE is rising, particularly in children. Although positive association between livestock production and human NDM-CRE infections at national level was identified, the evidence of direct transmission of NDM originating from livestock to humans remains largely unknown. Here we conducted a cross-sectional study in Chengdu, Sichuan Province to examine the prevalence of NDM-CRE in chickens and pigs along the breeding-slaughtering-retail chains, in pork in cafeterias of schools, and in colonisations and infections from a children's hospital, and examined the correlation of NDM-CRE among animals, foods and humans. Overall, the blaNDM increases gradually along the chicken and pig breeding (4.70%/2.0%) - slaughtering (7.60%/22.40%) - retail (65.56%/34.26%) chains. The slaughterhouse has become a hotspot for cross-contamination and amplifier of blaNDM. Notably, 63.11% pork from school cafeteria were positive for blaNDM. The prevalence of blaNDM in intestinal and infection samples from children's hospital was 21.68% and 19.80%, respectively. WGS analysis revealed the sporadic, not large-scale, clonal spread of NDM-CRE along the chicken and pig breeding-slaughtering-retail chain, with further spreading via IncX3-blaNDM plasmid within each stag...