Scaling up of a Self‐Confined Catalytic Hybridization Circuit for Robust microRNA Imaging

作者：Xue Gong, Ruomeng Li, Jiajia Zhang, Pu Zhang, Zhongwei Jiang, Lianzhe Hu, Xiaoqing Liu, Yi Wang, Fuan Wang · 发表于：Advanced Science · 年份：2024 · DOI：10.1002/advs.202400517 · 被引用次数：30 · 研究领域：Advanced biosensing and bioanalysis techniques、RNA Interference and Gene Delivery、Biosensors and Analytical Detection

The precise regulation of cellular behaviors within a confined, crowded intracellular environment is highly amenable in diagnostics and therapeutics. While synthetic circuitry system through a concatenated chemical reaction network has rarely been reported to mimic dynamic self-assembly system. Herein, a catalytic self-defined circuit (CSC) for the hierarchically concatenated assembly of DNA domino nanostructures is engineered. By incorporating pre-sealed symmetrical fragments into the preying hairpin reactants, the CSC system allows the hierarchical DNA self-assembly via a microRNA (miRNA)-powered self-sorting catalytic hybridization reaction. With minimal strand complexity, this self-sustainable CSC system streamlined the circuit component and achieved localization-intensified cascaded signal amplification. Profiting from the self-adaptively concatenated hybridization reaction, a reliable and robust method has been achieved for discriminating carcinoma tissues from the corresponding para-carcinoma tissues. The CSC-sustained self-assembly strategy provides a comprehensive and smart toolbox for organizing various hierarchical DNA nanostructures, which may facilitate more insights for clinical diagnosis and therapeutic assessment.