Duox activation in Drosophila Malpighian tubules stimulates intestinal epithelial renewal through a countercurrent flow

作者：Zhonggeng Liu, Hongyu Zhang, Bruno Lemaitre, Xiaoxue Li · 发表于：Cell Reports · 年份：2024 · DOI：10.1016/j.celrep.2024.114109 · 被引用次数：24 · 研究领域：Invertebrate Immune Response Mechanisms、Neurobiology and Insect Physiology Research、Insect symbiosis and bacterial influences

The gut must perform a dual role of protecting the host against toxins and pathogens while harboring mutualistic microbiota. Previous studies suggested that the NADPH oxidase Duox contributes to intestinal homeostasis in Drosophila by producing reactive oxygen species (ROS) in the gut that stimulate epithelial renewal. We find instead that the ROS generated by Duox in the Malpighian tubules leads to the production of Upd3, which enters the gut and stimulates stem cell proliferation. We describe in Drosophila the existence of a countercurrent flow system, which pushes tubule-derived Upd3 to the anterior part of the gut and stimulates epithelial renewal at a distance. Thus, our paper clarifies the role of Duox in gut homeostasis and describes the existence of retrograde fluid flow in the gut, collectively revealing a fascinating example of inter-organ communication.