Purine metabolism in lung adenocarcinoma: A single‐cell analysis revealing prognostic and immunotherapeutic insights

作者：Pengpeng Zhang, Shengbin Pei, Guangyao Zhou, Mengzhe Zhang, Lianmin Zhang, Zhenfa Zhang · 发表于：Journal of Cellular and Molecular Medicine · 年份：2024 · DOI：10.1111/jcmm.18284 · 被引用次数：30 · 研究领域：Single-cell and spatial transcriptomics、Cancer Genomics and Diagnostics、Cancer Immunotherapy and Biomarkers

Lung adenocarcinoma (LUAD) is a prevalent subtype of lung cancer, yet the contribution of purine metabolism (PM) to its pathogenesis remains poorly elucidated. PM, a critical component of intracellular nucleotide synthesis and energy metabolism, is hypothesized to exert a significant influence on LUAD development. Herein, we employed single-cell analysis to investigate the role of PM within the tumour microenvironment (TME) of LUAD. PM scoring (PMS) across distinct cell types was determined using AUCell, UCell, singscore and AddModuleScore algorithms. Subsequently, we explored communication networks among cells within high- and low-PMS groups, establishing a robust PM-associated signature (PAS) utilizing a comprehensive dataset comprising LUAD samples from TCGA and five GEO datasets. Our findings revealed that the high-PMS group exhibited intensified cell interactions, while the PAS, constructed using PM-related genes, demonstrated precise prognostic predictive capability. Notably, analysis across the TCGA dataset and five GEO datasets indicated that low-PAS patients exhibited a superior prognosis. Furthermore, the low-PAS group displayed increased immune cell infiltration and elevated CD8A expression, coupled with reduced PD-L1 expression. Moreover, data from eight publicly available immunotherapy cohorts suggested enhanced immunotherapy outcomes in the low-PAS group. These results underscore a close association between PAS and tumour immunity, offering predictive insights i...