Genome-wide association study and development of molecular markers for yield and quality traits in peanut (Arachis hypogaea L.)

作者：Minjie Guo, Li Deng, Jianzhong Gu, Jianli Miao, Junhua Yin, Yang Li, Yuanjin Fang, Bingyan Huang, Ziqi Sun, Feiyan Qi, Wenzhao Dong, Zhenhua Lü, Shaowei Li, Junping Hu, Xinyou Zhang, Li Ren · 发表于：BMC Plant Biology · 年份：2024 · DOI：10.1186/s12870-024-04937-5 · 被引用次数：24 · 研究领域：Peanut Plant Research Studies、Nuts composition and effects、Sunflower and Safflower Cultivation

BACKGROUND: This study aims to decipher the genetic basis governing yield components and quality attributes of peanuts, a critical aspect for advancing molecular breeding techniques. Integrating genotype re-sequencing and phenotypic evaluations of seven yield components and two grain quality traits across four distinct environments allowed for the execution of a genome-wide association study (GWAS). RESULTS: The nine phenotypic traits were all continuous and followed a normal distribution. The broad heritability ranged from 88.09 to 98.08%, and the genotype-environment interaction effects were all significant. There was a highly significant negative correlation between protein content (PC) and oil content (OC). The 10× genome re-sequencing of 199 peanut accessions yielded a total of 631,988 high-quality single nucleotide polymorphisms (SNPs), with 374 significant SNP loci identified in association with the nine traits of interest. Notably, 66 of these pertinent SNPs were detected in multiple environments, and 48 of them were linked to multiple traits of interest. Five loci situated on chromosome 16 (Chr16) exhibited pleiotropic effects on yield traits, accounting for 17.64-32.61% of the observed phenotypic variation. Two loci on Chr08 were found to be strongly associated with protein and oil contents, accounting for 12.86% and 14.06% of their respective phenotypic variations, respectively. Linkage disequilibrium (LD) block analysis of these seven loci unraveled five nonsynony...