Two orthogonal differentiation gradients locally coordinate fruit morphogenesis

作者：Andrea Gómez‐Felipe, Elvis Branchini, Binghan Wang, M. Marconi, Hana Bertrand-Rakusová, T. Stan, Jérôme Burkiewicz, Stefan de Folter, Anne‐Lise Routier‐Kierzkowska, Krzysztof Wabnik, Daniel Kierzkowski · 发表于：Nature Communications · 年份：2024 · DOI：10.1038/s41467-024-47325-1 · 被引用次数：29 · 研究领域：Plant Molecular Biology Research、Plant Reproductive Biology、Plant nutrient uptake and metabolism

Morphogenesis requires the coordination of cellular behaviors along developmental axes. In plants, gradients of growth and differentiation are typically established along a single longitudinal primordium axis to control global organ shape. Yet, it remains unclear how these gradients are locally adjusted to regulate the formation of complex organs that consist of diverse tissue types. Here we combine quantitative live imaging at cellular resolution with genetics, and chemical treatments to understand the formation of Arabidopsis thaliana female reproductive organ (gynoecium). We show that, contrary to other aerial organs, gynoecium shape is determined by two orthogonal, time-shifted differentiation gradients. An early mediolateral gradient controls valve morphogenesis while a late, longitudinal gradient regulates style differentiation. Local, tissue-dependent action of these gradients serves to fine-tune the common developmental program governing organ morphogenesis to ensure the specialized function of the gynoecium.