Development of an on‐site diagnostic LAMP assay for rapid differentiation of the invasive pest Phthorimaea absoluta (Meyrick) using insect tissues

作者：Li‐Feng Yang, Ya‐Ge Liu, Yun‐Li Tao, Weizhen Zhang, Jianyong Li, Shengqi Chi, Gui‐Fen Zhang, Dong Chu · 发表于：Pest Management Science · 年份：2024 · DOI：10.1002/ps.8114 · 被引用次数：9 · 研究领域：Insect behavior and control techniques、Insect Pheromone Research and Control、Biological Control of Invasive Species

BACKGROUND: The tomato leafminer, Phthorimaea absoluta (Meyrick) (Lepidoptera: Gelechiidae), is a destructive invasive pest that originated in South America and has spread within China since 2017. A rapid method for on-site identification of P. absoluta is urgently needed for interception of this pest across China. RESULTS: We developed a loop-mediated isothermal amplification (LAMP) technique to differentiate P. absoluta from Liriomyza sativae, Chromatomyia horticola, and Phthorimaea operculella using extracted genomic DNA, which was then refined to create an on-site LAMP diagnostic method that can be performed under field conditions without the need for laboratory equipment. CONCLUSION: In the present research, we developed an on-site diagnostic method for rapid differentiation of P. absoluta from other insects with similar morphology or damage characteristics in China. © 2024 Society of Chemical Industry.