Effect of MSX1 on the cellular function of cardiomyocytes

作者：Huang Linhuan, Liangying Zhong, Lin Shaobin, Zhu Caixia, Cai Danlei, Siqi Huang, Hong Peiming, Kong Shu, Xie Yingjun, Luo Yanmin · 发表于：Gene · 年份：2024 · DOI：10.1016/j.gene.2024.148419 · 被引用次数：2 · 研究领域：Cardiovascular Effects of Exercise、Mass Spectrometry Techniques and Applications、Genetics and Physical Performance

MSX1 (Muscle Segment Homeobox 1) has pleiotropic effects in various tissues, including cardiomyocytes, while the effect of MSX1 on cardiomyocyte cellular function was not well known. In this study, we used AC16 cell culture, real-time fluorescence quantitative PCR (qPCR), protein blotting (Western blot), flow cytometry apoptosis assay and lactate dehydrogenase (LDH) ELISA (Enzyme-Linked Immunosorbnent Assay) to investigate the effect of the MSX1 gene on cardiomyocyte function. The results showed that MSX1 plays a protective role against hypoxia of cardiomyocytes. However, further studies are required to fully understand the role of MSX1 in the regulation of LDH expression in different cell types and under different conditions.