Genomic and biological control of Sclerotinia sclerotiorum using an extracellular extract from Bacillus velezensis 20507

作者：Yunqing Cheng, Hanxiao Lou, Hongli He, Xinyi He, Zicheng Wang, Xin Gao, Jianfeng Liu · 发表于：Frontiers in Microbiology · 年份：2024 · DOI：10.3389/fmicb.2024.1385067 · 被引用次数：14 · 研究领域：Plant-Microbe Interactions and Immunity、Plant pathogens and resistance mechanisms、Plant Disease Management Techniques

Introduction Sclerotinia sclerotiorum is a known pathogen that harms crops and vegetables. Unfortunately, there is a lack of effective biological control measures for this pathogen. Bacillus velezensis 20507 has a strong antagonistic effect on S. Sclerotiorum ; however, the biological basis of its antifungal effect is not fully understood. Methods In this study, the broad-spectrum antagonistic microorganisms of B. velezensis 20507 were investigated, and the active antifungal ingredients in this strain were isolated, purified, identified and thermal stability experiments were carried out to explore its antifungal mechanism. Results The B. velezensis 20507 genome comprised one circular chromosome with a length of 4,043,341 bp, including 3,879 genes, 185 tandem repeats, 87 tRNAs, and 27 rRNAs. Comparative genomic analysis revealed that our sequenced strain had the closest genetic relationship with Bacillus velezensis (GenBank ID: NC 009725.2); however, there were significant differences in the positions of genes within the two genomes. It is predicted that B. velezensis 20507 encode 12 secondary metabolites, including difficidin, macrolactin H, fengycin, surfactin, bacillibactin, bacillothiazole A-N, butirosin a/b, and bacillaene. Results showed that B. velezensis 20507 produced various antagonistic effects on six plant pathogen strains: Exserohilum turcicum, Pyricularia oryzae, Fusarium graminearum, Sclerotinia sclerotiorum, Fusarium oxysporum , and Fusarium verticillioides . A...