The activation of iron deficiency responses of grapevine rootstocks is dependent to the availability of the nitrogen forms

作者：Sarhan Khalil, Rebeka Strah, Arianna Lodovici, Petr Vojta, Federica De Berardinis, Jörg Ziegler, Maruša Pompe‐Novak, Laura Zanin, Nicola Tomasi, Astrid Forneck, Michaela Griesser · 发表于：BMC Plant Biology · 年份：2024 · DOI：10.1186/s12870-024-04906-y · 被引用次数：9 · 研究领域：Plant Micronutrient Interactions and Effects、Horticultural and Viticultural Research、Plant Gene Expression Analysis

Abstract Background In viticulture, iron (Fe) chlorosis is a common abiotic stress that impairs plant development and leads to yield and quality losses. Under low availability of the metal, the applied N form (nitrate and ammonium) can play a role in promoting or mitigating Fe deficiency stresses. However, the processes involved are not clear in grapevine. Therefore, the aim of this study was to investigate the response of two grapevine rootstocks to the interaction between N forms and Fe uptake. This process was evaluated in a hydroponic experiment using two ungrafted grapevine rootstocks Fercal (Vitis berlandieri x V. vinifera ) tolerant to deficiency induced Fe chlorosis and Couderc 3309 ( V. riparia x V. rupestris ) susceptible to deficiency induced Fe chlorosis. Results The results could differentiate Fe deficiency effects, N-forms effects, and rootstock effects. Interveinal chlorosis of young leaves appeared earlier on 3309 C from the second week of treatment with NO 3 − /NH 4 + (1:0)/-Fe, while Fercal leaves showed less severe symptoms after four weeks of treatment, corresponding to decreased chlorophyll concentrations lowered by 75% in 3309 C and 57% in Fercal. Ferric chelate reductase (FCR) activity was by trend enhanced under Fe deficiency in Fercal with both N combinations, whereas 3309 C showed an increase in FCR activity under Fe deficiency only with NO 3 − /NH 4 + (1:1) treatment. With the transcriptome analysis, Gene Ontology (GO) revealed multiple biological p...