Development of a LC-QTOF-MS based dilute-and-shoot approach for the botanical discrimination of honeys

作者：Lei Tian, Shaghig Bilamjian, Lan Liu, Caren Akiki, Daniel J. Cuthbertson, Tarun Anumol, Stéphane Bayen · 发表于：Analytica Chimica Acta · 年份：2024 · DOI：10.1016/j.aca.2024.342536 · 被引用次数：14 · 研究领域：Bee Products Chemical Analysis、Insect and Pesticide Research、Pesticide Residue Analysis and Safety

Honeys of particular botanical origins can be associated with premium market prices, a trait which also makes them susceptible to fraud. Currently available authenticity testing methods for botanical classification of honeys are either time-consuming or only target a few "known" types of markers. Simple and effective methods are therefore needed to monitor and guarantee the authenticity of honey. In this study, a 'dilute-and-shoot' approach using liquid chromatography (LC) coupled to quadrupole time-of-flight-mass spectrometry (QTOF-MS) was applied to the non-targeted fingerprinting of honeys of different floral origin (buckwheat, clover and blueberry). This work investigated for the first time the impact of different instrumental conditions such as the column type, the mobile phase composition, the chromatographic gradient, and the MS fragmentor voltage (in-source collision-induced dissociation) on the botanical classification of honeys as well as the data quality. Results indicated that the data sets obtained for the various LC-QTOF-MS conditions tested were all suitable to discriminate the three honeys of different floral origin regardless of the mathematical model applied (random forest, partial least squares-discriminant analysis, soft independent modelling by class analogy and linear discriminant analysis). The present study investigated different LC-QTOF-MS conditions in a "dilute and shoot" method for honey analysis, in order to establish a relatively fast, simple and...