Enterococcus durans 98D alters gut microbial composition and function to improve DSS-induced colitis in mice

作者：Lei Yu, Yiting Yan, Junyu Zhong, Yitong Zhao, Yangbin Xu, Ting Zhang, Hui Xiong, Yulin Chen, Xiaolong Wang, Ke Zhang · 发表于：Heliyon · 年份：2024 · DOI：10.1016/j.heliyon.2024.e28486 · 被引用次数：8 · 研究领域：Gut microbiota and health、Probiotics and Fermented Foods、Helicobacter pylori-related gastroenterology studies

Enterococcus durans , is a potential functional strain with the capacity to regulate intestinal health and ameliorate colonic inflammation. However, the strain requires further investigation regarding its safety profile and potential mechanisms of colitis improvement. In this study, the safety of E. durans 98D (Ed) as a potential probiotic was studied using in vitro methods. Additionally, a dextran sulfate sodium (DSS)-induced murine colitis model was employed to investigate its impact on the intestinal microbiota and colitis. In vitro antimicrobial assays revealed Ed sensitivity to common antibiotics and its inhibitory effect on the growth of Escherichia coli O157, Streptococcus pneumoniae CCUG 37328, and Staphylococcus aureus ATCC 25923. To elucidate the functional properties of Ed, 24 weight-matched 6-week-old female C57BL/6J mice were randomly divided into three groups (n = 8): NC group, Con group (DSS), and Ed group (DSS + Ed). Ed administration demonstrated a protective effect on colitis mice, as evidenced by improvements in body weight, colonic length, reduced disease activity index, histological scores, diminished splenomegaly, and decreased goblet cell loss. Furthermore, Ed downregulated the expression of the pro-inflammatory cytokine genes ( IL-6 , IL-1β , and TNF-α ) and upregulated the expression of the anti-inflammatory cytokine gene IL-10 . The 16S rRNA gene sequencing revealed significant alterations in microbial α-diversity, with principal coordinate analysis ...