Resistance risk assessment for benzovindiflupyr in Sclerotium rolfsii and transmission of resistance genes among population

作者：Kaidi Cui, Chaofan Jiang, Long-Jiang Sun, Mengke Wang, Leiming He, Lin Zhou · 发表于：Pest Management Science · 年份：2024 · DOI：10.1002/ps.8101 · 被引用次数：21 · 研究领域：Fungal Plant Pathogen Control、Pesticide and Herbicide Environmental Studies、Plant Pathogens and Fungal Diseases

Abstract BACKGROUND Sclerotium rolfsii is a destructive soil‐borne fungal pathogen which is distributed worldwide. In previous study, the succinate dehydrogenase inhibitor (SDHI) fungicide benzovindiflupyr has been identified for its great antifungal activity against Sclerotium rolfsii . This study is aimed to investigate the resistance risk and mechanism of benzovindiflupyr in Sclerotium rolfsii . RESULTS Eight stable benzovindiflupyr‐resistant isolates were generated by fungicide adaptation. Although the obtained eight resistant isolates have a stronger pathogenicity than the parental sensitive isolate, they have a fitness penalty in the mycelial growth and sclerotia formation compared to the parental isolate. A positive cross‐resistance existed in the resistant isolates between benzovindiflupyr and thifluzamide, carboxin, boscalid and isopyrazam. Three‐point mutations, including SdhB N180D , SdhC Q68E and SdhD H103Y , were identified in the benzovindiflupyr‐resistant isolates. However, molecular docking analysis indicated that only SdhD H103Y could influence the sensitivity of Sclerotium rolfsii to benzovindiflupyr. After mycelial co‐incubation of resistant isolates and the sensitive isolate, resistance genes may be transmitted to the sensitive isolate. The in vivo efficacy of benzovindiflupyr and thifluzamide against benzovindiflupyr‐resistant isolates was a little lower than that against the sensitive isolate but with no significant difference. CONCLUSION The results sug...