A polyamino acid-based phosphatidyl polymer library for in vivo mRNA delivery with spleen targeting ability

作者：Hanqin Zhao, Sheng Ma, Yibo Qi, Yuxi Gao, Yuyan Zhang, Minhui Li, Jie Chen, Wantong Song, Xuesi Chen · 发表于：Materials Horizons · 年份：2024 · DOI：10.1039/d3mh02066e · 被引用次数：21 · 研究领域：RNA Interference and Gene Delivery、Advanced biosensing and bioanalysis techniques、Phagocytosis and Immune Regulation

studies proved that PLG-PPs/mRNA polyplexes exhibited a significant increase in mRNA expression, peaking 14 716 times compared to their non-phosphatidyl parent polymer. Impressively, the subset PA8-PL3 not only facilitated efficient mRNA transfection but also selectively delivered mRNA to the spleen instead of the liver (resulting in 69.73% protein expression in the spleen) once intravenously administered. This type of phosphatidyl PLG polymer library provides a novel approach to the construction of mRNA delivery systems especially for spleen-targeted mRNA therapeutic delivery.