Multi-epitope vaccine design for hepatitis E virus based on protein ORF2 and ORF3

作者：Qiong Lu, Hao Wu, Jing Meng, Jiangyuan Wang, Jiajing Wu, Shuo Liu, Jincheng Tong, Jianhui Nie, Weijin Huang · 发表于：Frontiers in Microbiology · 年份：2024 · DOI：10.3389/fmicb.2024.1372069 · 被引用次数：7 · 研究领域：Hepatitis Viruses Studies and Epidemiology、vaccines and immunoinformatics approaches、Hepatitis B Virus Studies

Introduction: Hepatitis E virus (HEV), with heightened virulence in immunocompromised individuals and pregnant women, is a pervasive threat in developing countries. A globaly available vaccine against HEV is currently lacking. Methods: We designed a multi-epitope vaccine based on protein ORF2 and ORF3 of HEV using immunoinformatics. Results: The vaccine comprised 23 nontoxic, nonallergenic, soluble peptides. The stability of the docked peptide vaccine-TLR3 complex was validated by molecular dynamic simulations. The induction of effective cellular and humoral immune responses by the multi-peptide vaccine was verified by simulated immunization. Discussion: These findings provide a foundation for future HEV vaccine studies.