Washed microbiota transplantation for Crohn’s disease: A metagenomic, metatranscriptomic, and metabolomic-based study

作者：Shi-Ju Chen, Daya Zhang, Xia Wu, Faming Zhang, Bota Cui, Yihao Huang, Zu-Lun Zhang, Rui Wang, Feihu Bai · 发表于：World Journal of Gastroenterology · 年份：2024 · DOI：10.3748/wjg.v30.i11.1572 · 被引用次数：8 · 研究领域：Gut microbiota and health、Clostridium difficile and Clostridium perfringens research、Inflammatory Bowel Disease

BACKGROUND Fecal microbiota transplantation (FMT) is a promising therapeutic approach for treating Crohn’s disease (CD). The new method of FMT, based on the automatic washing process, was named as washed microbiota transplantation (WMT). Most existing studies have focused on observing the clinical phenomena. However, the mechanism of action of FMT for the effective management of CD-particularly in-depth multi-omics analysis involving the metagenome, metatranscriptome, and metabolome-has not yet been reported. AIM To assess the efficacy of WMT for CD and explore alterations in the microbiome and metabolome in response to WMT. METHODS We conducted a prospective, open-label, single-center clinical study. Eleven CD patients underwent WMT. Their clinical responses (defined as a decrease in their CD Activity Index score of > 100 points) and their microbiome (metagenome, metatranscriptome) and metabolome profiles were evaluated three months after the procedure. RESULTS Seven of the 11 patients (63.6%) showed an optimal clinical response three months post-WMT. Gut microbiome diversity significantly increased after WMT, consistent with improved clinical symptoms. Comparison of the metagenome and metatranscriptome analyses revealed consistent alterations in certain strains, such as Faecalibacterium prausnitzii , Roseburia intestinalis , and Escherichia coli . In addition, metabolomics analyses demonstrated that CD patients had elevated levels of various amino acids before treatment ...