Population genetic variation and geographic distribution of suitable areas of Coptis species in China

作者：Yujie Chi, Changli Liu, Wei Liu, Xufang Tian, Juan Hu, Bó Wáng, Di Liu, Yifei Liu · 发表于：Frontiers in Plant Science · 年份：2024 · DOI：10.3389/fpls.2024.1341996 · 被引用次数：7 · 研究领域：Genetic diversity and population structure、Species Distribution and Climate Change、Forest, Soil, and Plant Ecology in China

Introduction The rhizomes of Coptis plants have been used in traditional Chinese medicine over 2000 years. Due to increasing market demand, the overexploitation of wild populations, habitat degradation and indiscriminate artificial cultivation of Coptis species have severely damaged the native germplasms of species in China. Methods Genome-wide simple-sequence repeat (SSR) markers were developed using the genomic data of C. chinensis . Population genetic diversity and structure of 345 Coptis accessions collected from 32 different populations were performed based on these SSRs. The distribution of suitable areas for three taxa in China was predicted and the effects of environmental variables on genetic diversity in relation to different population distributions were further analyzed. Results 22 primer pairs were selected as clear, stable, and polymorphic SSR markers. These had an average of 16.41 alleles and an average polymorphism information content (PIC) value of 0.664. In the neighbor-joining (N-J) clustering analysis, the 345 individuals clustered into three groups, with C. chinensis , C. chinensis var. brevisepala and C. teeta being clearly separated. All C. chinensis accessions were further divided into four subgroups in the population structure analysis. The predicted distributions of suitable areas and the environmental variables shaping these distributions varied considerably among the three species. Discussion Overall, the amount of solar radiation, precipitation an...