Antithrombotic pharmacodynamics and metabolomics study in raw and processed products of Whitmania pigra Whitman

作者：Hongqian Kui, Lei Yan, Chunxue Jia, Quan‐Cheng Xin, Rustam Tursun, Miao Zhong, Chuanxin Liu, Ruijuan Yuan · 发表于：Heliyon · 年份：2024 · DOI：10.1016/j.heliyon.2024.e27828 · 被引用次数：6 · 研究领域：Leech Biology and Applications、Marine Invertebrate Physiology and Ecology、Healthcare and Venom Research

Objective As a traditional Chinese medicine, leech has obvious pharmacological activities in anticoagulantion and antithrombosis. Whitmania pigra Whitman (WP) is the most commonly used leech in the Chinese market. It is often used in clinical applications after high-temperature processing by talcum powder to remove the fishy taste and facilitate crushing. The anticoagulant and thrombolytic active ingredients are protein and polypeptide, which may denaturate and lose activity after high-temperature processing. The rationality of its processing has been questioned in recent years. This study aims to investigate the effect of talcum powder scalding on the antithrombotic activity of WP in vivo and to discuss its pharmacodynamic mechanism in vivo . Methods Raw and talcum-powdered processed WP were administered intragastrically for 14 days, and carrageenan was injected intraperitoneally to prepare a mouse model of tail vein thrombosis. The incidence rate of tail vein thrombosis and the thrombus area under pathological tissue sections were calculated to evaluate the antithrombotic effect between raw and processed WP. Non-targeted metabolomics was conducted using UPLC-QTOF/MS technology to analyze the changes of small molecule metabolites in the body after administration of WP. Results After intragastric administration, both the raw product and the processed product of WP could inhibit the thrombosis induced by carrageenan, and the processed product had a more apparent antithrombotic...