Paeoniflorin Alleviates Anxiety and Visceral Hypersensitivity via HPA Axis andBDNF/TrkB/PLCγ1 Pathway in Maternal Separation-induced IBS-like Rats

作者：Ruifeng Liang, Wenjing Ge, Song Xian-mei, Huisen Wang, Weifeng Cui, Xuexia Zhang, Wei Zheng, LI Geng-sheng · 发表于：Current Molecular Pharmacology · 年份：2024 · DOI：10.2174/0118761429280572240311060851 · 被引用次数：15 · 研究领域：Gastrointestinal motility and disorders、Stress Responses and Cortisol、Congenital gastrointestinal and neural anomalies

BACKGROUND: Irritable Bowel Syndrome (IBS) is a prevalent gastrointestinal disorder that significantly diminishes the quality of life for affected individuals. The pathophysiology of IBS remains poorly understood, and available therapeutic options for IBS are limited. The crucial roles of brain-gut interaction, which is mediated by the Hypothalamic-Pituitary-Adrenocortical (HPA) axis and the autonomic nervous system in IBS, have attracted increasing attention. OBJECTIVE: The objective of this study was to examine the impact of paeoniflorin (PF) on anxiety and visceral hypersensitivity in maternal separation-induced IBS-like rats. METHODS: The IBS-like rat model was established through the implementation of Maternal Separation (MS) and subsequently subjected to various doses of PF administered via oral gavage for 14 days. Anxiety-like behavior was evaluated using the Open Field Test (OFT) and Elevated Plus Maze (EPM) test. The assessment of visceral sensitivity involved the utilization of the Abdominal Withdrawal Reflex (AWR) score and electromyographic (EMG) responses of the external oblique muscle in response to colorectal distention. The levels of adrenocorticotropic hormone (ACTH), corticosterone (CORT), and corticotrophin-releasing hormone (CRH) were examined by ELISA. Quantitative real-time PCR (qRT-PCR) and immunofluorescence were employed to detect the expressions of CRH receptors 1 (CRHR1) and 2 (CRHR2). Glucocorticoid receptors (GR), mineralocorticoid receptor (MR), ...