Physiological and Transcriptional Characteristics of Banana Seedlings in Response to Nitrogen Deficiency Stress

作者：Lei Zhao, Bingyu Cai, Xiaohan Zhang, Bencheng Zhang, Junting Feng, Dengbo Zhou, Yufeng Chen, Miaoyi Zhang, Dengfeng Qi, Wei Wang, Jianghui Xie, Yongzan Wei · 发表于：Horticulturae · 年份：2024 · DOI：10.3390/horticulturae10030290 · 被引用次数：12 · 研究领域：Plant nutrient uptake and metabolism、Plant Micronutrient Interactions and Effects、Growth and nutrition in plants

Nitrogen is a crucial element for the growth and development of plants, directly affecting crop growth and yield. To investigate the physiological and molecular mechanism of nitrogen-deficiency stress, we conducted an investigation into the effects of different nitrogen levels on the growth, photosynthetic characteristics, and gene transcription levels of banana seedlings. Compared with the control group with normal nitrogen levels (NN), the height of plants receiving Reduced-N (NR), Low-N (LN), and N-Free (NF) treatments was decreased by 0.45 cm, 2.5 cm, and 3.25 cm, respectively. Their dry weight was reduced by 1.63 g, 2.99 g, and 2.88 g, respectively. Conversely, the dry weight of the underground plant part in the LN and NF treatment groups exhibited an increase of 0.13 g and 0.16 g, respectively. Regarding photosynthetic characteristics, the Specialty Products Agricultural Division (SPAD) values of the NR, LN, and NF treatments showed reductions of 15.5%, 30.4%, and 35.9%, respectively, compared with those of the control treatments. The values of maximum photosynthetic efficiency (Fv/Fm), actual photosynthetic efficiency (Y(Ⅱ)), and relative electron transfer (ETR) of the banana seedlings decreased to different degrees after NR, LN, and NF treatment, and their values were positively correlated with N levels. Gene transcription analysis showed that N transport-related proteins, including NRT1.7, NRT2.3a, NRT2.3b, and NRT2.5, were significantly up-regulated to increase the ...