Plant growth regulators mitigate oxidative damage to rice seedling roots by NaCl stress

作者：Yaxin Wang, Liming Zhao, Naijie Feng, Dianfeng Zheng, Xue Shen, Hang Zhou, Wenxin Jiang, Youwei Du, Huimin Zhao, Xutong Lu, Peng Deng · 发表于：PeerJ · 年份：2024 · DOI：10.7717/peerj.17068 · 被引用次数：12 · 研究领域：Plant Stress Responses and Tolerance、Photosynthetic Processes and Mechanisms、Plant responses to elevated CO2

The aim of this experiment was to investigate the effects of exogenous sprays of 5-aminolevulinic acid (5-ALA) and 2-Diethylaminoethyl hexanoate (DTA-6) on the growth and salt tolerance of rice ( Oryza sativa L.) seedlings. This study was conducted in a solar greenhouse at Guangdong Ocean University, where ‘Huanghuazhan’ was selected as the test material, and 40 mg/L 5-ALA and 30 mg/L DTA-6 were applied as foliar sprays at the three-leaf-one-heart stage of rice, followed by treatment with 0.3% NaCl (W/W) 24 h later. A total of six treatments were set up as follows: (1) CK: control, (2) A: 40 mg⋅ L −1 5-ALA, (3) D: 30 mg⋅ L −1 DTA-6, (4) S: 0.3% NaCl, (5) AS: 40 mg⋅ L −1 5-ALA + 0.3% NaCl, and (6) DS: 30 mg⋅ L −1 DTA-6+0.3% NaCl. Samples were taken at 1, 4, 7, 10, and 13 d after NaCl treatment to determine the morphology and physiological and biochemical indices of rice roots. The results showed that NaCl stress significantly inhibited rice growth; disrupted the antioxidant system; increased the rates of malondialdehyde, hydrogen peroxide, and superoxide anion production; and affected the content of related hormones. Malondialdehyde content, hydrogen peroxide content, and superoxide anion production rate significantly increased from 12.57% to 21.82%, 18.12% to 63.10%, and 7.17% to 56.20%, respectively, in the S treatment group compared to the CK group. Under salt stress, foliar sprays of both 5-ALA and DTA-6 increased antioxidant enzyme activities and osmoregulatory substance ...