Acanthopanax senticosus improves cognitive impairment in Alzheimer’s disease by promoting the phosphorylation of the MAPK signaling pathway

作者：Zhichun Zhang, Yonghui Wu, Dan Shi, Chanyu Jiang, Hengyan Cao, Fengyi Jiang, Xiaomin Bao, Yan Shen, Shi Xiao · 发表于：Frontiers in Immunology · 年份：2024 · DOI：10.3389/fimmu.2024.1383464 · 被引用次数：14 · 研究领域：Natural product bioactivities and synthesis、Medicinal Plants and Neuroprotection、Ziziphus Jujuba Studies and Applications

Background Acanthopanax senticosus (AS) can improve sleep, enhance memory, and reduce fatigue and is considered as an effective drug for Alzheimer’s disease (AD). The therapeutic effect and mechanism need to be further investigated. Methods To confirm the AS play efficacy in alleviating memory impairment in mice, 5×FAD transgenic mice were subjected to an open-field experiment and a novelty recognition experiment. Network pharmacology technique was used to analyze the information of key compounds and potential key targets of AS for the treatment of AD, molecular docking technique was applied to predict the binding ability of targets and compounds, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were also performed on the targets to derive the possible metabolic processes and pathway mechanisms of AS in treating AD. Quantitative real-time PCR (qRT-PCR) and western blot technique were carried out to validate the candidate genes and pathways. Results In the open-field experiment, compared with the wild-type (WT) group, the number of times the mice in the AD group crossed the central zone was significantly reduced ( P < 0.01). Compared with the AD group, the number of times the mice in the AS group crossed the central zone was significantly increased ( P < 0.001). In the new object recognition experiment, compared with the WT group, the percentage of times the AD group explored new objects was significantly reduced ( P < 0.0...