H 2 S preconditioning induces long-lived perturbations in O 2 metabolism

作者：David A. Hanna, Jutta Diessl, Arkajit Guha, Roshan Kumar, Anthony C. Andren, Costas Andreas Lyssiotis, Ruma V. Banerjee · 发表于：Proceedings of the National Academy of Sciences · 年份：2024 · DOI：10.1073/pnas.2319473121 · 被引用次数：18 · 研究领域：Sulfur Compounds in Biology、Neuroscience of respiration and sleep、Heme Oxygenase-1 and Carbon Monoxide

Hydrogen sulfide exposure in moderate doses can induce profound but reversible hypometabolism in mammals. At a cellular level, H 2 S inhibits the electron transport chain (ETC), augments aerobic glycolysis, and glutamine-dependent carbon utilization via reductive carboxylation; however, the durability of these changes is unknown. We report that despite its volatility, H 2 S preconditioning increases P 50(O2) , the O 2 pressure for half-maximal cellular respiration, and has pleiotropic effects on oxidative metabolism that persist up to 24 to 48 h later. Notably, cyanide, another complex IV inhibitor, does not induce this type of metabolic memory. Sulfide-mediated prolonged fractional inhibition of complex IV by H 2 S is modulated by sulfide quinone oxidoreductase, which commits sulfide to oxidative catabolism. Since induced hypometabolism can be beneficial in disease settings that involve insufficient or interrupted blood flow, our study has important implications for attenuating reperfusion-induced ischemic injury and/or prolonging the shelf life of biologics like platelets.