Dual transcriptomic and metabolomic analyses provide novel insights into the role of vitamins A and B metabolism in ectomycorrhizal symbiosis between Pinus yunnanensis and Lactarius deliciosus

作者：Kai Su, Jing Yuan, Ran Wang, Lan-Lan Huang, Jian Zhan, Xueqiong Zhang, Shanping Wan, Xinhua He, Hans Lambers, Fuqiang Yu, Yanliang Wang · 发表于：Physiologia Plantarum · 年份：2024 · DOI：10.1111/ppl.14194 · 被引用次数：10 · 研究领域：Mycorrhizal Fungi and Plant Interactions、Plant Parasitism and Resistance、Fungal Biology and Applications

Abstract Ectomycorrhizal (EM) fungi play important roles in nutrient cycling and plant community establishment in forest ecosystems. Effects of EM formation on global alterations of the transcriptome and metabolome during plant‐fungal interaction and the key metabolites involved in EM development are largely unknown. Here, dual RNA‐Seq and untargeted metabolomic analyses were used to reveal stage‐specific and core responses of Pinus yunnanensis and Lactarius deliciosus during mycorrhizal colonization. We found that L. deliciosus colonization in P. yunnanensis roots induced different transcriptional changes across three interaction stages, with a small core of genes consistently regulated at all stages. Concentrations of retinol (vitamin A) and retinoic acid increased while that of B group vitamins decreased during EM formation, which was coordinately regulated by these two plant‐fungus partners, with L. deliciosus possibly playing a dominant role. Exogenous retinol altered the diameter and mantle thickness of P. yunnanensis ‐ L. deliciosus EM tips and affected host plant growth and phosphorus acquisition . In the absence of L. deliciosus, exogenous retinol increased the root diameter and the number of root tips of P. yunnanensis . Furthermore, the concentration of auxin increased, but that of abscisic acid decreased during EM formation, and the genes involved in plant hormone signal transduction were gradually activated, and auxin and cytokinin signal transduction potentially...