Environmental DNA time series analysis of a temperate stream reveals distinct seasonal community and functional shifts

作者：Mandy Sander, Arne J. Beermann, Dominik Buchner, Iris Madge Pimentel, James S. Sinclair, Martina Weiss, Peter Haase, Florian Leese · 发表于：River Research and Applications · 年份：2024 · DOI：10.1002/rra.4265 · 被引用次数：22 · 研究领域：Environmental DNA in Biodiversity Studies、Microbial Community Ecology and Physiology、Protist diversity and phylogeny

Abstract Environmental DNA (eDNA) extracted from water is routinely used in river biodiversity research, and via metabarcoding eDNA can provide comprehensive taxa lists with little effort and cost. However, eDNA‐based species detection in streams and rivers may be influenced by sampling season and other key factors such as water temperature and discharge. Research linking these factors and also informing on the potential of eDNA metabarcoding to detect shifts in ecological signatures, such as species phenology and functional feeding groups across seasons, is missing. To address this gap, we collected water samples every 2 weeks for 15 months at a long‐term ecological research (LTER) site and at three different positions in the river's cross section, specifically the water surface, riverbed, and riverbank. For these 102 samples, we analyzed macroinvertebrate species and molecular operational taxonomic unit (OTU) richness and temporal community turnover across seasons based on cytochrome c oxidase subunit I (COI) metabarcoding data. Using Generalized Additive Models, we found a significant influence of sampling season on species richness. Community turnover followed a cyclic pattern, reflecting the continuous change of the macroinvertebrate community throughout the year (“seasonal clock”). Although water temperature had no influence on the inferred species richness, higher discharge reduced the number of Annelida and Ephemeroptera species detectable with eDNA. Most macroinverte...