Pilot-scale acetone-butanol-ethanol fermentation from corn stover

作者：Changsheng Su, Di Cai, Honggang Zhang, Yilu Wu, Yongjie Jiang, Yicheng Liu, Changwei Zhang, Chunling Li, Peiyong Qin, Tianwei Tan · 发表于：Green Carbon · 年份：2024 · DOI：10.1016/j.greenca.2024.02.004 · 被引用次数：26 · 研究领域：Biofuel production and bioconversion、Microbial Metabolic Engineering and Bioproduction、Enzyme Catalysis and Immobilization

Biobutanol is an advanced biofuel that can be produced from excess lignocellulose via acetone-butanol-ethanol (ABE) fermentation. Although significant technological progress has been made in this field, attempts at large-scale lignocellulosic ABE production remain scarce. In this study, 1 m3 scale ABE fermentation was investigated using high inhibitor tolerance Clostridium acetobutylicum ABE-P1201 and steam-exploded corn stover hydrolysate (SECSH). Before expanding the fermentation scale, the detoxification process for SECSH was simplified by process engineering. Results revealed that appropriate pH management during the fed-batch cultivation could largely decrease the inhibition of the toxic components in undetoxified SECSH to the solventogenesis phase of the ABE-P1201 strains, avoiding ‘acid crash’. Therefore, after naturalizing the pH by Ca(OH)2, the undetoxified SECSH, without removal of the solid components, reached 17.68±1.30 g/L of ABE production with 0.34±0.01 g/g of yield in 1 L scale bioreactor. Based on this strategy, the fermentation scale gradually expanded from laboratory-scale apparatus to pilot-scale bioreactors. Finally, 17.05±1.20 g/L of ABE titer and 0.32±0.01 g/g of ABE yield were realized in 1 m3 bioreactor, corresponding to approximately 145 kg of ABE production from 1 t of dry corn stover. The pilot-scale ABE fermentation demonstrated excellent stability during repeated operations. This study provided a simplified ABE fermentation strategy and verified ...