A mouse DRG genetic toolkit reveals morphological and physiological diversity of somatosensory neuron subtypes

作者：Lijun Qi, Michael Iskols, David E. Shi, Pranav Reddy, Christopher J. Walker, Karina Lezgiyeva, Tiphaine Voisin, Mathias Pawlak, Vijay K. Kuchroo, Isaac M. Chiu, David D. Ginty, Nikhil Sharma · 发表于：Cell · 年份：2024 · DOI：10.1016/j.cell.2024.02.006 · 被引用次数：187 · 研究领域：Neurobiology and Insect Physiology Research、Ion channel regulation and function、Neuroscience and Neuropharmacology Research

Dorsal root ganglia (DRG) somatosensory neurons detect mechanical, thermal, and chemical stimuli acting on the body. Achieving a holistic view of how different DRG neuron subtypes relay neural signals from the periphery to the CNS has been challenging with existing tools. Here, we develop and curate a mouse genetic toolkit that allows for interrogating the properties and functions of distinct cutaneous targeting DRG neuron subtypes. These tools have enabled a broad morphological analysis, which revealed distinct cutaneous axon arborization areas and branching patterns of the transcriptionally distinct DRG neuron subtypes. Moreover, in vivo physiological analysis revealed that each subtype has a distinct threshold and range of responses to mechanical and/or thermal stimuli. These findings support a model in which morphologically and physiologically distinct cutaneous DRG sensory neuron subtypes tile mechanical and thermal stimulus space to collectively encode a wide range of natural stimuli.