Evaluation and genetic dissection of the powdery mildew resistance in 558 wheat accessions

作者：Yuli Jin, Guohao Han, Wenjing Zhang, Bin Bu, Ya Zhao, Jiaojiao Wang, Ruishan Liu, Hong Gang Yang, Hongxing Xu, Pengtao Ma · 发表于：New Crops · 年份：2024 · DOI：10.1016/j.ncrops.2024.100018 · 被引用次数：16 · 研究领域：Wheat and Barley Genetics and Pathology、Plant Disease Resistance and Genetics、Plant pathogens and resistance mechanisms

Powdery mildew, a widespread and destructive wheat disease caused by the fungal pathogen Blumeria graminis f. sp. tritici (Bgt), results in significant yield losses globally. Employing host resistance is the most cost-effective and environmentally sustainable approach to managing this disease. Assessing the resistance levels of wheat germplasms and understanding the genetic underpinnings of this resistance is crucial. In this study, we evaluated the seedling resistance to powdery mildew in 558 wheat accessions, along with the presence of resistance genes Pm1, Pm2, Pm4, Pm5, Pm6, Pm8, Pm12, Pm21, Pm24, Pm41, Pm42, Pm45, Pm47, Pm60, and Pm69 using various Bgt isolates and associated diagnostic markers. The study revealed that out of 558 wheat accessions tested, only 25 showed resistance to the Bgt isolate E09, highlighting a general lack of powdery mildew resistance among the evaluated accessions. Furthermore, these 25 accessions exhibited a significantly different resistance spectrum to 25 additional Bgt isolates. Notably, seven of these accessions were resistant to all the tested isolates, indicating they possess Pm genes with broad-spectrum resistance. Molecular analysis using closely linked or diagnostic markers revealed diverse resistance gene profiles: 249 accessions harbored a single tested Pm gene, 75 accessions possessed combinations of Pm genes, and 234 accessions lacked any of the tested genes. Pm8 was the most frequently detected gene, present in 27.42% of accession...