Tissue-specific regulation of functional compensation effect of AiPC4s' knockdown reduces thermotolerance in scallop Argopecten irradians irradians

作者：Ancheng Liu, Xiujiang Hou, Junhao Zhang, Ao Wang, Zhi Liu, Wen Wang, Xuecheng Dong, Longfei Chu, Jiaxi Chang, Xinghai Zhu, Qiang Xing, Xiaoting Huang, Jingjie Hu, Zhenmin Bao · 发表于：Aquaculture · 年份：2024 · DOI：10.1016/j.aquaculture.2024.740736 · 被引用次数：6 · 研究领域：Physiological and biochemical adaptations、Ocean Acidification Effects and Responses、Genetics, Aging, and Longevity in Model Organisms

Transcriptional coactivator p15 ( PC4 ) encodes an effectively diverse protein with highly conserved structure and performs critical functions in RNA polymerase II-mediated transcription, DNA replication and damage repair. Although comprehensive investigations of PC4 have been characterized in most vertebrates and some invertebrates, the crucial roles of PC4 were less systematically validated in bivalve mollusks . In the present study, the function of PC4 , particularly Arg0230340.1 which had been identified to be highly associated to thermotolerance , in bay scallops Argopecten irradians irradians ( AiPC4s ) was verified through suppression of Arg0230340.1 expression with dsRNA-mediated RNAi (5 μM, 10 μM and 20 μM). Then, expression regulations of AiPC4s in temperature sensitive tissues (heart, mantle and gill) and cardiac performance including resting heart rate (RHR), maximum heart rate (HR max ) and Arrhenius break temperature (ABT) of bay scallops were detected 24 h after injection of siRNA . After interference, Arg0230340.1 displayed an obvious decrease in experimental groups (5 μM, 10 μM and 20 μM) relative to that in control (NG) and blank groups (DG). Simultaneously, the suppression of Arg0230340.1 expression resulted in a significant decrease of HR max in experimental groups, but with no marked differences for RHR among groups. Importantly, the suppression of Arg0230340.1 significantly weakened the thermotolerance of bay scallops, as proven by a reduction up to 4.09...