Aligned Nanofibers Promote Myoblast Polarization and Myogenesis through Activating Rac-Related Signaling Pathways

作者：Xiangyu Dong, Shan Su, Qian Sun, Peng Wang, Qian Hu, Qiang Wei · 发表于：ACS Biomaterials Science & Engineering · 年份：2024 · DOI：10.1021/acsbiomaterials.4c00127 · 被引用次数：14 · 研究领域：Muscle Physiology and Disorders、Cellular Mechanics and Interactions、Electrospun Nanofibers in Biomedical Applications

The extracellular matrix (ECM) plays a crucial role in regulating cellular behaviors and functions. However, the impact of ECM topography on muscle cell adhesion and differentiation remains poorly understood from a mechanosensing perspective. In this study, we fabricated aligned and random electrospun polycaprolactone (PCL) nanofibers to mimic the structural characteristics of ECM. Mechanism investigations revealed that the orientation of nanofibers promoted C2C12 polarization and myogenesis through Rac-related signaling pathways. Conversely, cells cultured on random fibers exhibited spreading behavior mediated by RhoA/ROCK pathways, resulting in enhanced stress fiber formation but reduced capacity for myogenic differentiation. Our findings highlight the critical role of an ECM structure in muscle regeneration and damage repair, providing novel insights into mechanosensing mechanisms underlying muscle injury diseases.