A Dual Fluorescence Turn‐On Sensor Array Formed by Poly( para ‐aryleneethynylene) and Aggregation‐Induced Emission Fluorophores for Sensitive Multiplexed Bacterial Recognition

作者：Yang Yu, Weiwei Ni, Qin Hu, Huihai Li, Yi Zhang, Xu Gao, Lingjia Zhou, Shu-Ming Zhang, Shuoyang Ma, Yanliang Zhang, Hui Huang, Fei Li, Jinsong Han · 发表于：Angewandte Chemie International Edition · 年份：2024 · DOI：10.1002/anie.202318483 · 被引用次数：72 · 研究领域：Luminescence and Fluorescent Materials、Advanced biosensing and bioanalysis techniques、Polydiacetylene-based materials and applications

Abstract Bacterial infections have emerged as the leading causes of mortality and morbidity worldwide. Herein, we developed a dual‐channel fluorescence “turn‐on” sensor array, comprising six electrostatic complexes formed from one negatively charged poly( para ‐aryleneethynylene) (PPE) and six positively charged aggregation‐induced emission (AIE) fluorophores. The 6‐element array enabled the simultaneous identification of 20 bacteria (OD 600 =0.005) within 30s (99.0 % accuracy), demonstrating significant advantages over the array constituted by the 7 separate elements that constitute the complexes. Meanwhile, the array realized different mixing ratios and quantitative detection of prevalent bacteria associated with urinary tract infection (UTI). It also excelled in distinguishing six simulated bacteria samples in artificial urine. Remarkably, the limit of detection for E. coli and E. faecalis was notably low, at 0.000295 and 0.000329 (OD 600 ), respectively. Finally, optimized by diverse machine learning algorithms, the designed array achieved 96.7 % accuracy in differentiating UTI clinical samples from healthy individuals using a random forest model, demonstrating the great potential for medical diagnostic applications.